Evidencia de actividad feromonal de contacto en Cerambyx welensii Küster y compuestos candidatos

  1. López Manzano, M.R. 1
  2. Sánchez Osorio, I. 1
  3. López Pantoja, G. 1
  4. Domínguez, L. 1
  5. Rosell, G. 2
  6. Guerrero, A. 2
  1. 1 Departamento de Ciencias Agroforestales, Escuela Técnica Superior de Ingeniería, Universidad de Huelva
  2. 2 Departamento de Química Biológica y Modelización Molecular, Instituto de Química Avanzada de Cataluña, CSIC
Journal:
Cuadernos de la Sociedad Española de Ciencias Forestales

ISSN: 1575-2410,  2386-8368

Year of publication: 2020

Issue: 46

Pages: 17-32

Type: Article

DOI: 10.31167/CSECFV0I46.19896 DIALNET GOOGLE SCHOLAR lock_openDialnet editor

More publications in: Cuadernos de la Sociedad Española de Ciencias Forestales

Abstract

The aim of this work was to determine the existence of contact sex pheromone in Cerambyx welensii (Coleoptera, Cerambycidae). Solid phase micro extraction samples from elitrum and prothorax of C. welensii males and females were analyzed by CG-MS. Additional bioassays were performed to confirm the existence of contact pheromone in the cuticle of C. welensii females. For this purpose, the response of males was checked to: intact females, dead (freeze-killed) females, dead females after cuticular waxes were removed by hexane rinsing, and females covered with their own cuticular hexane extract. An amount of 44 cuticular hydrocarbons were found in elytra and 17 in prothorax. In both elytra and prothorax, the same 6 compounds together comprised between 68.1% (male eliters) and 85.4% (female prothorax) of the whole hydrocarbon content. None of the compounds were specific to females, but differences between sexes were found in the relative presence for some compounds. The candidate compounds to act as a contact pheromone could be located both in elytra and prothorax, with 11-Me-C29, C27, 2-Me-C26 and 11/13-Me-C27 being proposed to potentially act as major components (relative abundances > 17%), whiles 11/12/13-Me-C26 and C28 could be proposed as candidates to act as minor components (relative abundance <2.5%). The majority of males (82%) showed the characteristic sequence of mating-behavior stages: Antenal contact - Dashing - Licking - Abdominal bending. Males exhibit similar responses to dead females, but not to solvent-washed dead females with their cuticular hydrocarbons removed (occurrence of either licking and abdominal bending <5%). The mating response of males was partially restored when solvent extracts were reapplied to carcasses of solvent-washed females (22% of positive responses), indicating that the contact pheromone was present in solvent extracts.

Bibliographic References

  • Allison, J.D., Borden, J.H., Seybold, J.H., 2004. A review of the chemical ecology of the Cerambycidae (Coleoptera). Chemoecology 14: 123-150. https://doi.org/10.1007/s00049-004-0277-1
  • Barbour, J., Lacey, E., Hanks, L., 2007. Cuticular hydrocarbons mediate mate recognition in a species of longhorned beetle (Coleoptera: Cerambycidae) of the primitive subfamily prioninae. Ann. Entomol. Soc. Am. 100: 333-338. https://doi.org/10.1603/0013-8746(2007)100[333:CHMMRI]2.0.CO;2
  • Buse, J., Schröderb, B., Assmann, T., 2007. Modelling habitat and spatial distribution of an endangered longhorn beetle - A case study for saproxylic insect conservation. Biol. Conserv. 137: 372-381. https://doi.org/10.1016/j.biocon.2007.02.025
  • Carrasco, A., y cols., 2009. Procesos de Decaimiento Forestal (la Seca), Situación del Conocimiento. Consejería de Medio Ambiente, Junta de Andalucía, 112 pp. Córdoba.
  • Chiang, Y.N., Tan, K.J., Chung, H., Lavrynenko, O., Shevchenko, A., Yew, J.Y., 2016. Steroid hormone signaling is essential for pheromone production and oenocyte survival. PLoS Genet. 12: e1006126. https://doi.org/10.1371/journal.pgen.1006126
  • de la Cruz, J., 2006. Entomología, Morfología y Fisiología de los Insectos (pp. 33-126). Palmira: Editorial Universidad Nacional de Colombia.
  • del Moral, J., 1994. Cerambyx spp.: historia de una plaga de las dehesas extremeñas, Phytoma-España, 60: 18-24.
  • Duque-Lazo, J., Navarro-Cerrillo, R., 2017. What to save, the host or the pest? The spatial distribution of xylophage insects within the Mediterranean oak woodlands of Southwestern Spain. For. Ecol. Manag 92: 90-104. https://doi.org/10.1016/j.foreco.2017.02.047
  • Fukaya, M., Akino, T., Yasuda, T., Tatsuki, S., Wakamura, S., 1999. Mating sequence and evidence for synergistic component in female contact sex pheromone of the white-spotted longicorn beetle, Anoplophora malasiaca (Thomson) (Coleoptera: Cerambycidae). Entomol. Sci. 2: 183-187.
  • Ginzel, M., 2010. Hydrocarbons as contact pheromones of longhorned beetles (Coleoptera: Cerambycidae). In: Blomquist, G., Bagnères, A., (eds.), Insect Hydrocarbons: Biology, Biochemistry, and Chemical Ecology (pp. 375-389). Cambridge: Cambridge University Press. https://doi.org/10.1017/CBO9780511711909.018
  • Ginzel, M.D., Blomquist, G.J., Millar, J.G., 2003a. Role of contact pheromones in mate recognition in Xylotrechus colonus. J. Chem. Ecol. 29: 533-545.
  • Ginzel, M.D., Millar, J.G., Hanks, L.M., 2003b. (Z)-9-Pentacosene - contact sex pheromone of the locust borer, Megacyllene robiniae. Chemoecol. 13:135-141. https://doi.org/10.1007/s00049-003-0239-z
  • Ginzel, M.D., Blomquist, G., 2016. Insect Hydrocarbons: Biochemistry and Chemical Ecology. In: Cohen, E., Moussian, B. (eds.), Extracellular composite matrices in arthropods. Switzerland: Springer International Publishing. pp. 221-252. https://doi.org/10.1007/978-3-319-40740-1_7
  • Ibeas, F., Gemeno, C., Díez, J.J., Pajares, J.A., 2008. Female recognition and sexual dimorphism of cuticular hydrocarbons in Monochamus galloprovincialis (Coleoptera: Cerambycidae). Ann. Entomol. Soc. Am. 102: 317-325. https://doi.org/10.1603/008.102.0214
  • Lacey, E., Ginzel, M., Millar, J., Hanks, L., 2008. 7‐methylheptacosane is a major component of the contact sex pheromone of the cerambycid beetle Neoclytus acuminatus acuminatus. Physiol. Entomol. 33: 209-2016. https://doi.org/10.1111/j.1365-3032.2008.00624.x
  • Lockey, K., 1985. Insect cuticular lipids. Comp. Biochem. Physiol. B 81: 263-267. https://doi.org/10.1016/0305-0491(85)90311-6
  • Lopes, O., Marques, P., Araújo, J., 2005. The role of antennae in mate recognition in Phoracantha semipunctata (Coleoptera: Cerambycidae). J. Insect Behav. 18: 243-257. https://doi.org/10.1007/s10905-005-0478-7
  • López-Pantoja, G., Domínguez, L., Sánchez-Osorio, I., 2008. Mark-recapture estimates of the survival and recapture rates of Cerambyx welensii Küster (Coleoptera Cerambycidae) in a cork oak dehesa in Huelva (Spain). Cent. Eur. J. Biol. 3: 431-441. https://doi.org/10.2478/s11535-008-0044-3
  • Martín, J., Cabezas, J., Buyolo, T., Patón, D., 2005. The relationship between Cerambyx spp. damage and subsequent Biscogniauxia mediterranum infection on Quercus suber forests. For. Ecol. Manag. 216: 166-174. https://doi.org/10.1016/j.foreco.2005.05.027
  • Navarro, R.M., Fernández, P., 2001. Evaluation de dégats produits par le déperisement du chêne-liege en Andalusie. Selviculture of cedar (Cedrus atlantica End. M.) and cork oak (Quercus suber L.). IUFRO.
  • Pajares, J.A., Álvarez, G., Ibeas, F., Gallego, D., Hall, D.R., Farman, D.I., 2010. Identification and field activity of male-produced aggregation pheromone in the pine sawyer beetle, Monochamus galloprovincialis. J. Chem. Ecol. 36: 570-583. https://doi.org/10.1007/s10886-010-9791-5
  • Rodríguez, A., 2014. Mejora en las estrategias de control de cerambícidos en el cultivo de la vid. Universidad de León. 338 pp
  • Sacristán, A., Sánchez-Husillos, E., Hall, DR., Pajares, JA., 2017. Feromonas de contacto implicadas en el reconocimiento sexual en Monochamus galloprovincialis y Monochamus sutor (Coleoptera: Cerambycidae). Panel presentado en el 7º Congreso Forestal Español, Plasencia.
  • Sallé, A., Nageleisen, L.M., Lieutier, F., 2014. Bark and wood boring insects involved in oak declines in Europe: current knowledge and future prospects in a context of climate change. For. Ecol. Manag. 328: 79-93. https://doi.org/10.1016/j.foreco.2014.05.027
  • Sánchez-Osorio, I. Tapias, R., López, G., Domínguez, L., 2012. Pautas de apareamiento y localización intraespecífica en Cerambyx welensii Küster (Coleoptera: Cerambycidae). Cuad. Soc. Esp. Cienc. For. 36: 119-125.
  • Sánchez-Osorio, I., López-Pantoja, G., Paramio, A.M., Lencina, J.L., Gallego, D., Domínguez, L,. 2016. Field attraction of Cerambyx welensii to fermentation odors and host monoterpenes. J. Pest. Sci. 89: 59-68. https://doi.org/10.1007/s10340-015-0654-2
  • Sánchez-Osorio, I., López-Pantoja, G., Tapias, R., Pareja-Sánchez, E., Domínguez, L., 2019. Monoterpene emission of Quercus suber L. highly infested by Cerambyx welensii Küster. Ann. For. Sci. 89. https://doi.org/10.1007/s13595-019-0879-y
  • Silk, P.J., Sweeney, J., Wu, J., Sopow, S., Mayo, P.D., Magee, D., 2011. Contact sex pheromones identified for two species of longhorned beetles (Coleoptera: Cerambycidae) Tetropium fuscum and T. cinnamopterum in the Subfamily Spondylidinae. Env. Entomol. 40: 714-726. https://doi.org/10.1603/EN10213
  • Soria, F.J., Villagrán, M., Ocete, M., 1994. Estudios prospectivos de los principales perforadores del alcornoque en la Sierra Norte de Sevilla. Bol. San.Veg. Plagas 20: 643-651.
  • Tiberi, R., Branco, M., Bracalini, M., Croci, F., Panzavolta, T., 2016. Cork oak pests: a review of insect damage and management. Ann. For. Sci. https://doi.org/10.1007/s13595-015-0534-1
  • Torres-Vila, L.M., Sanchez-González, A., Ponce-Escudero, F., Martín-Vertedor, D., Ferrero-García, J.J., 2012. Assessing mass trapping efficiency and population density of Cerambyx welensii Küster by mark-recapture in dehesa open woodlands. Eur. J. Forest. Res. 131: 1103-1116. https://doi.org/10.1007/s10342-011-0579-0
  • Wang, Q., 2002. Sexual selection of Zorion guttigerum Westwood (Coleoptera: Cerambycidae) in relation to body size and color. J. Insect Behav. 15: 701-713.
  • Wang, Q., Zeng, W., Chen, L., Li, J., Yin, X., 2002. Circadian reproductive rhytms, pair bonding, and evidence for sex specific pheromones in Nadezhdiella cantori, J. Insect Behav. 15: 527-539. https://doi.org/10.1023/A:1016333317564
  • WWW/Adena, 2006. La dehesa en los Programas de desarrollo rural 2007-13. Propuesta. 34pp.
Data source: Dialnet