BEHAVIOR OF Ag NANOPARTICLES (Ag-NPs) DURING ECOTOXICOLOGICAL TEST ON GILTHEAD SEABREAM LARVAE

  1. M.J. Casanueva Marenco 2
  2. G. Fernández-López 2
  3. M. Yahia 1
  4. M. Abdelrahim 1
  5. C.J. Sánchez-Vazquez 2
  6. J.J. García-Guzmán 1
  7. J.M. Palacios-Santander 1
  8. M.D. Granado Castro 2
  9. L. Cubillana-Aguilera 1
  10. M. Díaz de Alba 2
  11. A. Canalejo Raya 3
  12. F. Córdoba García 3
  13. R. Torronteras Santiago 3
  14. M.D. Galindo Riaño 2
  1. 1 Department of Analytical Chemistry, Institute of Electronic Microscopy and Materials (IMEYMAT). Faculty of Science, CEI-MAR. University of Cadiz. Spain.
  2. 2 Department of Analytical Chemistry. Institute of Biomolecules (INBIO), Faculty of Science, CEI-MAR. University of Cadiz. Spain
  3. 3 Department of Environmental Biology and Public Health. Faculty of Experimental Sciences, CEI-MAR. University of Huelva. Spain.
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Actas:
International Conference on Environmental and Food Monitoring (ISEAC-40)

Editorial: Universidad de Santiago de Compostela y Universidad de Zaragoza

ISBN: 978-84-09-03799-5

Año de publicación: 2018

Páginas: 355

Tipo: Aportación congreso

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Resumen

Nanomaterials (NMs) are increasingly entering the environment with uncertain consequences. Ecotoxicological tests are used for risk assessment of pollutants but the specific behavior and properties of nanomaterials are not usually considered in the bioassays. It is frequently the failure to consider the information about the nanomaterial type and characteristics, the interaction of NM with the matrix of the test medium and the dispersion procedure performed to prepare the test medium. The difficulties in dose metrics may lead results that are difficult to compare. Also, the use of environmentally relevant concentrations, where observing outcomes is difficult, is not usually used versus higher NMs doses, where responses are easily observable. Thus, the approach of these issues seems necessary. In this work, the behavior and evolution of silver nanoparticles (Ag-NPs) with the test medium of versus ionic silver (Ag+) during an ecotoxicological test on sea bream larvae (Sparus Aurata) were studied. The organisms were exposed to different concentrations of Ag-NP and ionic silver: 0 (control); 0.001; 0.01; 0.1 and 1 mg L-1 at different exposure times: 24 to 72 hours with a 50% daily water changes. Laboratory conditions with oxygen-saturated water at 20 ºC and 12/12 dark/light cycle were used. The behavior of the Ag-NP and ionic silver in tanks (where appropriate) was monitoring at exposure times by: a) the measurement of actual metal concentration by ICP-MS analysing the total metal, the 0.45 and 0.22 µm filtered fractions, and the ultra-centrifuged fraction; b) the spectrum of test media by UV/Vis spectroscopy; c) the measurement of particle size distribution by dynamic light scatering; d) the study of centrifuged solids by scanning and transmission electron microscopy (SEM and TEM).